Blue Taq DNA polymerase is a mixture of LeGene DnaUs Taq DNA Polymerase and an inert blue dye. The blue dye has no effect on PCR amplification or downstream application including sequencing and cloning. However, it can be easily removed using a standard purification method if desired. DnaUs Taq DNA Polymerase is purrified from E.coli. expressing a cloned Thurmus aquaticus DNA polymerase gene. This enzyme has an intrinsic 5’->3’ DNA polymerase and a 5’->3’ exonuclease activity but lacks a 3’->5’ exonuclease activity. It is a single polypeptide chain with a molecular weight of approximately 95 kDa and displays optimal activity at temperature between 70-74oC. The enzyme is provided with 10XPCR buffer contains 15mM MgCl2 solution to perform PCR amplification.
