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Fr-Fr qPCR Master Mix, ROX (5X), 50 X 50 μl reactions

Price: $71.00
Catalog Number: 6682-05
Requires Dry Ice Shipping
Protocols and Manuals

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Fr-Fr qPCR Master Mix, ROX is a ready-to-use reaction cocktail that contains all components except primers and template for real-time quantitative PCR in systems that do not require ROX. The proprietary buffer and stabilizers have been optimized to deliver maximum PCR efficiency, sensitivity and robust fluorescent signal. The proprietary 5X qPCR Master Mix contains DnaUs Hot Start Taq DNA polymerase, dNTPs, Mg2+, enhancer, stabilizer and an optimal concentration of ROX for instruments that require ROX for a reference signal. Please consult our website and Instrument Compatibility section below for the correct product for your real-time PCR system. DnaUs Hot Start Taq DNA polymerase is an antibody-inactivated hot-start enzyme designed to block polymerase activity at ambient temperature. Once the PCR step reaches denaturation temperature (95oC), Taq DNA polymerase activity is restored and the resulting PCR exhibits higher sensitivity, specificity and yield. The system enables highly sensitive detection from few copies of DNA template, with a broad dynamic range that supports accurate quantification of target sequences. Sufficient reagents are provided for 50 amplification reactions of 50 μl each.