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LeGene SB-Green qPCR Master Mix, ROX, 500 X 50 μl reactions (10 X 1.25 ml)
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Catalog Number: 6442-50
Requires Dry Ice Shipping
* Whole number only
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Protocols and Manuals
Description
LeGene SB-Green qPCR Master Mix, ROX is a ready-to-use reaction cocktail that contains all components except primers and template for real-time quantitative PCR on
systems that require ROX input. The proprietary buffer and stabilizers have been optimized for SYBR Green qPCR to deliver maximum PCR efficiency, sensitivity
and robust fluorescent signal. The proprietary 2X qPCR Master Mix, ROX contains DnaUs Hot Start Taq DNA polymerase, SYBR Green 1 Dye, dNTPs, Mg2+, enhancer, and stabilizer
and an optimal concentration of ROX for instruments that require ROX for a reference signal.
DnaUs Hot Start Taq DNA polymerase is an antibody-inactivated hot-start enzyme designed to block polymerase activity at ambient temperature. Once the PCR step
reaches denaturation temperature (95oC), Taq DNA polymerase activity is restored and the resulting PCR exhibits higher sensitivity, specificity and yield.
The system enables highly sensitive detection from few copies of DNA template, with a broad dynamic range that supports accurate quantification of target sequences.
Sufficient reagents are provided for 500 amplification reactions of 50 μl each.
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