Glycerol-free RnaUsScript Reverse Transcriptase, 20,000 units (200,000 units/ml)

Protocols and Manuals

Catalog No. 6160-20: RnaUsScript Reverse Transcriptase, Size: 20,000 U

Description

Glycero-Free RnaUsScript Reverse Transcriptase (RNaseH Minus) is an engineered from Moloney Murine Leukemia Virus RT to reduce RNaseH activity and enhance cDNA yield. RnaUsScript RT is a single polypeptide chain with a molecular weight of approximately 78 kDa, is an RNA-dependent DNA polymerase that synthesizes the complementary cDNA strand from a single-stranded RNA template to which a primer has been annealed (1,2). RnaUsScript RT also efficiently extend primers hybridized to single-stranded DNA. This enzyme is expressed in E. coli (2) and purified to free of exogenous RNase activity, a common contaminant in commercial preparations of reverse transcriptase.The enzyme is used to synthesize first-strand cDNA up to 12 kb and 5X cDNA Synthesis buffer is provided.