|
Glycerol-free RnaUsScript-Gold Reverse Transcriptase, 100,000 units (200,000 units/ml)
|
Catalog Number: 6157-70
Requires Dry Ice Shipping
|
Protocols and Manuals
Catalog No. 6157-70: Glycerol-free RnaUsScript-Gold Reverse Transcriptase, Size: 100,000 U
Description
Glycerol-Free RnaUsScript-Gold Reverse Transcriptase (RNaseH Minus) is an engineered
from Moloney Murine Leukemia Virus RT to eliminate RNaseH activity and enhance cDNA yield at
an elevated temperature up to 60 oC. Glycerol-Free RnaUsScript-Gold RT is a single polypeptide
chain with a molecular weight of approximately 78.2 kDa, is an RNA-dependent DNA polymerase that
synthesizes the complementary cDNA strand from a single-stranded RNA template to which a primer
has been annealed (1,2). Glycerol-Free RnaUsScript-Gold RT also efficiently extend primers
hybridized to single-stranded DNA. This enzyme is expressed in E. coli (2) and purified to free
of exogenous RNase activity, a common contaminant in commercial preparations of reverse transcriptase.
The enzyme is used to synthesize first-strand cDNA up to 13 kb and 5X cDNA Synthesis buffer is provided.
|
|
|