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RnaUsScript-Gold Reverse Transcriptase, 20,000 units (200,000 units/ml)
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Catalog Number: 6159-20
Requires Dry Ice Shipping
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Protocols and Manuals
Catalog No. 6159-20: RnaUsScript-Gold Reverse Transcriptase, Size: 20,000 U
Description
RnaUsScript-Gold Reverse Transcriptase (RNaseH Minus) is an engineered from Moloney Murine
Leukemia Virus RT to eliminate RNaseH activity and enhance cDNA yield at an elevated temperature
up to 60 oC..RnaUsScript-Gold RT is a single polypeptide chain with a molecular weight of
approximately 78.2 kDa, is an RNA-dependent DNA polymerase that synthesizes the complementary
cDNA strand from a single-stranded RNA template to which a primer has been annealed (1,2).
RnaUsScript-Gold RT also efficiently extend primers hybridized to single-stranded DNA.
This enzyme is expressed in E. coli (2) and purified to free of exogenous RNase activity,
a common contaminant in commercial preparations of reverse transcriptase. The enzyme is
used to synthesize first-strand cDNA up to 13 kb and 5X cDNA Synthesis buffer is provided.
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